Expressing:Article Title: Slp2-a controls renal epithelial cell size through regulation of Rap-ezrin signaling independently of Rab27.
Article Snippet: DBA/2JJcl mouse kidneys and pcy mouse kidneys were purchased from CLEA Japan Inc. (Tokyo, Japan) and KYUDO Co. (Saga, Japan), respectively. .. pEGFP-C1 (Clontech-Takara Bio, Shiga, Japan), pmRFP-C1 and/or pEFT7 tag expression vectors harboring cDNAs of mouse Slp2-a and Rab27A and their mutants were prepared essentially as described previously (Fukuda and Kuroda, 2002; Kuroda and Fukuda, 2004). cDNAs encoding human Rap1A, Rap2A, Rap1GAP1 and Rap1GAP2, and mouse ezrin were amplified from Marathon-Ready adult human or mouse brain and testis cDNA (Clontech-Takara Bio), and Drosophila Btsz was amplified from a Drosophila cDNA library (Merck Biosciences Novagen) by PCR with the following pairs of oligonucleotides having a restriction enzyme site (underlined) as described previously (Fukuda et al., 1999): 59-GGATCCATGCGTGAGTACAAGCTAGT-39 and 59-GTCGACCTAGAGCAGCAGACATGATT-39 for Rap1A, 59-GGATCCATGCGCGAGTACAAAGTGGT-39 and 59-GTCGACCTATTGTATGTTACATGCAG-39 for Rap2A, 59-GGATCCATGAGCGGCCGGAAGCGCA-39 and 59-CTAACAGCCCAGCTGGGGCAT-39 for Rap1GAP1, 59-AGATCTATGTTTGGCCGGAAGCGCAG-39 and 59- GTCGACTTAGTGACCCGCACCAGAGC-39 for Rap1GAP2, 59-GGATCCTCGCACAGTGGGGGCATCCCTGGC-39 and 59-GTCGACCTAGCTGGGGCTCTCGCTGCTCGG-39 for RGDN, 59-AGATCTCCACCATGCCCAAGCCAATCAACG-39 and 59-GGTACCCGGGCCTGGGCCTTCATCTGCTG-39 for the N-terminal domain of ezrin, 59- AGATCTGCCACCATGAAGGCCCAGGCCCGG-39 and 59-GGTACCCATGGCCTCGAACTCGTCAATGCG-39 for the C-terminal domain of ezrin, and 59-GGATCCATGGAAGCCAAAGTGGCCGCT-39 and 59- GTCGACCTATGGCAAATTGGCTCGAATGC-39 for Btsz. ..
Amplification:Article Title: Slp2-a controls renal epithelial cell size through regulation of Rap-ezrin signaling independently of Rab27.
Article Snippet: DBA/2JJcl mouse kidneys and pcy mouse kidneys were purchased from CLEA Japan Inc. (Tokyo, Japan) and KYUDO Co. (Saga, Japan), respectively. .. pEGFP-C1 (Clontech-Takara Bio, Shiga, Japan), pmRFP-C1 and/or pEFT7 tag expression vectors harboring cDNAs of mouse Slp2-a and Rab27A and their mutants were prepared essentially as described previously (Fukuda and Kuroda, 2002; Kuroda and Fukuda, 2004). cDNAs encoding human Rap1A, Rap2A, Rap1GAP1 and Rap1GAP2, and mouse ezrin were amplified from Marathon-Ready adult human or mouse brain and testis cDNA (Clontech-Takara Bio), and Drosophila Btsz was amplified from a Drosophila cDNA library (Merck Biosciences Novagen) by PCR with the following pairs of oligonucleotides having a restriction enzyme site (underlined) as described previously (Fukuda et al., 1999): 59-GGATCCATGCGTGAGTACAAGCTAGT-39 and 59-GTCGACCTAGAGCAGCAGACATGATT-39 for Rap1A, 59-GGATCCATGCGCGAGTACAAAGTGGT-39 and 59-GTCGACCTATTGTATGTTACATGCAG-39 for Rap2A, 59-GGATCCATGAGCGGCCGGAAGCGCA-39 and 59-CTAACAGCCCAGCTGGGGCAT-39 for Rap1GAP1, 59-AGATCTATGTTTGGCCGGAAGCGCAG-39 and 59- GTCGACTTAGTGACCCGCACCAGAGC-39 for Rap1GAP2, 59-GGATCCTCGCACAGTGGGGGCATCCCTGGC-39 and 59-GTCGACCTAGCTGGGGCTCTCGCTGCTCGG-39 for RGDN, 59-AGATCTCCACCATGCCCAAGCCAATCAACG-39 and 59-GGTACCCGGGCCTGGGCCTTCATCTGCTG-39 for the N-terminal domain of ezrin, 59- AGATCTGCCACCATGAAGGCCCAGGCCCGG-39 and 59-GGTACCCATGGCCTCGAACTCGTCAATGCG-39 for the C-terminal domain of ezrin, and 59-GGATCCATGGAAGCCAAAGTGGCCGCT-39 and 59- GTCGACCTATGGCAAATTGGCTCGAATGC-39 for Btsz. ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). .. cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S-transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously (24). .. Plasmid Construction—cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCRwith the following specific oligonucleotides as described previously (25): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/ MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593– 858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). .. Plasmid Construction cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: .. Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
cDNA Library Assay:Article Title: Slp2-a controls renal epithelial cell size through regulation of Rap-ezrin signaling independently of Rab27.
Article Snippet: DBA/2JJcl mouse kidneys and pcy mouse kidneys were purchased from CLEA Japan Inc. (Tokyo, Japan) and KYUDO Co. (Saga, Japan), respectively. .. pEGFP-C1 (Clontech-Takara Bio, Shiga, Japan), pmRFP-C1 and/or pEFT7 tag expression vectors harboring cDNAs of mouse Slp2-a and Rab27A and their mutants were prepared essentially as described previously (Fukuda and Kuroda, 2002; Kuroda and Fukuda, 2004). cDNAs encoding human Rap1A, Rap2A, Rap1GAP1 and Rap1GAP2, and mouse ezrin were amplified from Marathon-Ready adult human or mouse brain and testis cDNA (Clontech-Takara Bio), and Drosophila Btsz was amplified from a Drosophila cDNA library (Merck Biosciences Novagen) by PCR with the following pairs of oligonucleotides having a restriction enzyme site (underlined) as described previously (Fukuda et al., 1999): 59-GGATCCATGCGTGAGTACAAGCTAGT-39 and 59-GTCGACCTAGAGCAGCAGACATGATT-39 for Rap1A, 59-GGATCCATGCGCGAGTACAAAGTGGT-39 and 59-GTCGACCTATTGTATGTTACATGCAG-39 for Rap2A, 59-GGATCCATGAGCGGCCGGAAGCGCA-39 and 59-CTAACAGCCCAGCTGGGGCAT-39 for Rap1GAP1, 59-AGATCTATGTTTGGCCGGAAGCGCAG-39 and 59- GTCGACTTAGTGACCCGCACCAGAGC-39 for Rap1GAP2, 59-GGATCCTCGCACAGTGGGGGCATCCCTGGC-39 and 59-GTCGACCTAGCTGGGGCTCTCGCTGCTCGG-39 for RGDN, 59-AGATCTCCACCATGCCCAAGCCAATCAACG-39 and 59-GGTACCCGGGCCTGGGCCTTCATCTGCTG-39 for the N-terminal domain of ezrin, 59- AGATCTGCCACCATGAAGGCCCAGGCCCGG-39 and 59-GGTACCCATGGCCTCGAACTCGTCAATGCG-39 for the C-terminal domain of ezrin, and 59-GGATCCATGGAAGCCAAAGTGGCCGCT-39 and 59- GTCGACCTATGGCAAATTGGCTCGAATGC-39 for Btsz. ..
Polymerase Chain Reaction:Article Title: Slp2-a controls renal epithelial cell size through regulation of Rap-ezrin signaling independently of Rab27.
Article Snippet: DBA/2JJcl mouse kidneys and pcy mouse kidneys were purchased from CLEA Japan Inc. (Tokyo, Japan) and KYUDO Co. (Saga, Japan), respectively. .. pEGFP-C1 (Clontech-Takara Bio, Shiga, Japan), pmRFP-C1 and/or pEFT7 tag expression vectors harboring cDNAs of mouse Slp2-a and Rab27A and their mutants were prepared essentially as described previously (Fukuda and Kuroda, 2002; Kuroda and Fukuda, 2004). cDNAs encoding human Rap1A, Rap2A, Rap1GAP1 and Rap1GAP2, and mouse ezrin were amplified from Marathon-Ready adult human or mouse brain and testis cDNA (Clontech-Takara Bio), and Drosophila Btsz was amplified from a Drosophila cDNA library (Merck Biosciences Novagen) by PCR with the following pairs of oligonucleotides having a restriction enzyme site (underlined) as described previously (Fukuda et al., 1999): 59-GGATCCATGCGTGAGTACAAGCTAGT-39 and 59-GTCGACCTAGAGCAGCAGACATGATT-39 for Rap1A, 59-GGATCCATGCGCGAGTACAAAGTGGT-39 and 59-GTCGACCTATTGTATGTTACATGCAG-39 for Rap2A, 59-GGATCCATGAGCGGCCGGAAGCGCA-39 and 59-CTAACAGCCCAGCTGGGGCAT-39 for Rap1GAP1, 59-AGATCTATGTTTGGCCGGAAGCGCAG-39 and 59- GTCGACTTAGTGACCCGCACCAGAGC-39 for Rap1GAP2, 59-GGATCCTCGCACAGTGGGGGCATCCCTGGC-39 and 59-GTCGACCTAGCTGGGGCTCTCGCTGCTCGG-39 for RGDN, 59-AGATCTCCACCATGCCCAAGCCAATCAACG-39 and 59-GGTACCCGGGCCTGGGCCTTCATCTGCTG-39 for the N-terminal domain of ezrin, 59- AGATCTGCCACCATGAAGGCCCAGGCCCGG-39 and 59-GGTACCCATGGCCTCGAACTCGTCAATGCG-39 for the C-terminal domain of ezrin, and 59-GGATCCATGGAAGCCAAAGTGGCCGCT-39 and 59- GTCGACCTATGGCAAATTGGCTCGAATGC-39 for Btsz. ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). .. cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). .. Plasmid Construction cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: .. Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Plasmid Preparation:Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S-transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously (24). .. Plasmid Construction—cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCRwith the following specific oligonucleotides as described previously (25): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/ MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593– 858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). .. Plasmid Construction cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
Affinity Purification:Article Title: Rabex-5 Protein Regulates Dendritic Localization of Small GTPase Rab17 and Neurite Morphogenesis in Hippocampal Neurons
Article Snippet: .. Anti-Rabex-5 rabbit polyclonal antibody was raised against glutathione S -transferase (GST)-Rabex5-C (amino acid residues (aa) 208–491) and affinity purified by exposure to antigen-bound Affi-Gel 10 beads (Bio-Rad) as described previously ( 24 ). cDNAs encoding the human DENN domain-containing proteins, mouse VPS9 domain-containing proteins, and mouse Sec2 domain-containing proteins were amplified from Marathon-Ready adult human or mouse brain and/or testis cDNA (Clontech-Takara Bio Inc., Shiga, Japan) by performing PCR with the following specific oligonucleotides as described previously ( 25 ): DENN/MADD/Rab3-GEP (aa 1–570), DENND1A (aa 1–380), DENND1B (aa 5–410), DENND1C (aa 1–424), DENND2A (aa 551–1009), DENND2B (aa 669–1237), DENND2C (aa 392–881), DENN2D (aa 1–471), DENND3 (aa 24–435), DENND4A (aa 139–660), DENND4B (aa 99–650), DENND4C (aa 1–420), DENND5A (aa 1–599), DENND5B (aa 18–600), DENND6A (aa 1–605), DENND6B (aa 1–585), Sbf1/MTMR5 (aa 1–467), Sbf2/MTMR13 (aa 1–585), Varp (aa 1–450), Rabex-5 (aa 208–491), Gapex-5 (aa 1281–1437), Rin1 (aa 446–763), Rin2 (aa 593–858), Rin3 (aa 713–980), ALS2 (aa 1485–1651), ALS2-cl (aa 764–947), RINL (aa 314–563), Rabin3 (aa 1–428), and GRAB (aa 1–384). ..
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